Journal: Anatomy Physiology & Biochemistry International Journal

Article Title: The Effect of Pulsed Electromagnetic Field Stimulation of Live Cells on Intracellular Ca2+ Dynamics Changes Notably Involving Ion Channels

doi: 10.19080/apbij.2023.06.555710

Figure Lengend Snippet: Figure 1: PEMF Stimulation promotes ATP-induced intracellular Ca2+ ([Ca2+] i) increase in KG-1 cells. (A) Representative traces showing combined each ATP-induced [Ca2+] i transients from individual single cells in control (without PEMF stimulation) and PEMF stimulated cells. (B) Representative traces showing that averaged (Mean ± SEM.) [Ca2+]i response induced by ATP. (C) Graph illustrating the mean net increases in [Ca2+]i revealed by the averaged intensity obtained from three different PEMF stimulation conditions versus control. (D) mRNA expression of P2 type of purinergic receptors were amplified by reverse transcription-polymerase chain reaction (RT-PCR) using the control KG-1 cells. Original gel showing expression levels of seven P2X subtypes and six P2Y subtypes. (E) Images showing morphology of adherent KG-1 cells and regions of interest for analysis (upper, left). PEMF-generating device and control system with 6-channels magnetic coils (upper, right). Schematic design of the PEMF system (lower). The coils were placed in the CO2 incubator for cell stimulation. Experiments were performed more than three times in each condition. Asterisks indicate *P < 0.05, ***P < 0.001.

Article Snippet: Cell culture and PEMF exposure KG-1 (Korean Cell Line Bank, Seoul, Korea) cells were cultured in RPMI1640 medium (Croning, New York, USA) containing 10 % heat inactivated fetal bovine serum (Cytiva, Marlborough, USA) and 50 U/ml Penicillin, 50 ug/ml streptomycin (Thermo Fisher, Waltham, USA).

Techniques: Control, Expressing, Amplification, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Cell Stimulation

Journal: Anatomy Physiology & Biochemistry International Journal

Article Title: The Effect of Pulsed Electromagnetic Field Stimulation of Live Cells on Intracellular Ca2+ Dynamics Changes Notably Involving Ion Channels

doi: 10.19080/apbij.2023.06.555710

Figure Lengend Snippet: Figure 2: PEMF stimulation promotes cytoplasmic store Ca2+ release and store-operated Ca2+ entry in KG-1 cells. (A) Representative traces showing [Ca2+] i response induced by store depletion using CPA in the absence of extracellular Ca2+ in control cells, followed Ca2+ influx into the cells by increasing extracellular Ca2+. (B) Traces showing comparison of averaged (Mean ± SEM.) [Ca2+] i response in control and PEMF stimulated cells. (C) Graph illustrating the mean net increases in [Ca2+] i obtained by CPA and extracellular high Ca2+ change. (D) Original gel showing significant levels of Orai1, Orai2, Orai3, Stim1, and Stim2. Asterisks indicate **P < 0.01.

Article Snippet: Cell culture and PEMF exposure KG-1 (Korean Cell Line Bank, Seoul, Korea) cells were cultured in RPMI1640 medium (Croning, New York, USA) containing 10 % heat inactivated fetal bovine serum (Cytiva, Marlborough, USA) and 50 U/ml Penicillin, 50 ug/ml streptomycin (Thermo Fisher, Waltham, USA).

Techniques: Control, Comparison

Journal: Anatomy Physiology & Biochemistry International Journal

Article Title: The Effect of Pulsed Electromagnetic Field Stimulation of Live Cells on Intracellular Ca2+ Dynamics Changes Notably Involving Ion Channels

doi: 10.19080/apbij.2023.06.555710

Figure Lengend Snippet: Figure 3: PEMF Stimulation represses ATP-induced [Ca2+] i increase in HUVECs. (A) Representative traces showing combined each ATP-induced [Ca2+] i transients from individual single cells in control and PEMF stimulated cells. (B) Representative traces showing that averaged (Mean ± SEM.) [Ca2+] i response induced by ATP. (C) Graph illustrating the mean net increases of ATP-induced [Ca2+] i from PEMF stimulation condition versus control. (D) Original gel showing expression level of genes which seven subtypes of P2X and six subtypes of P2Y. (E) Showing the morphologies of HUVECs at 100X magnification and comparing the ATP effects between control (upper) and PEMF treated (lower) cells (○, □; before ATP, / ●, ■; after ATP). Asterisks indicate ***P < 0.001.

Article Snippet: Cell culture and PEMF exposure KG-1 (Korean Cell Line Bank, Seoul, Korea) cells were cultured in RPMI1640 medium (Croning, New York, USA) containing 10 % heat inactivated fetal bovine serum (Cytiva, Marlborough, USA) and 50 U/ml Penicillin, 50 ug/ml streptomycin (Thermo Fisher, Waltham, USA).

Techniques: Control, Expressing

Journal: Anatomy Physiology & Biochemistry International Journal

Article Title: The Effect of Pulsed Electromagnetic Field Stimulation of Live Cells on Intracellular Ca2+ Dynamics Changes Notably Involving Ion Channels

doi: 10.19080/apbij.2023.06.555710

Figure Lengend Snippet: Figure 4: PEMF stimulation represses cytoplasmic store Ca2+ release in HUVECs. (A) Representative traces showing [Ca2+] i response induced by store depletion using CPA in the absence of extracellular Ca2+ in control and PEMF stimulated cells. (B) Representative traces showing that averaged (Mean ± SEM.) [Ca2+] i response induced by CPA. (C) Graph illustrating the mean net increases in [Ca2+] i obtained by CPA from PEMF stimulation condition versus control. (D) Original gel showing significant levels of Orai1, Orai2, Orai3, Stim1, and Stim2. Asterisks indicate ***P < 0.001.

Article Snippet: Cell culture and PEMF exposure KG-1 (Korean Cell Line Bank, Seoul, Korea) cells were cultured in RPMI1640 medium (Croning, New York, USA) containing 10 % heat inactivated fetal bovine serum (Cytiva, Marlborough, USA) and 50 U/ml Penicillin, 50 ug/ml streptomycin (Thermo Fisher, Waltham, USA).

Techniques: Control